- Title
- Rhodococcus wratislaviensis strain 9: an efficient p-nitrophenol degrader with a great potential for bioremediation
- Creator
- Subashchandrabose, Suresh R.; Venkateswarlu, Kadiyala; Krishnan, Kannan; Naidu, Ravi; Lockington, Robin; Megharaj, Mallavarapu
- Relation
- Journal of Hazardous Materials Vol. 347, p. 176-183
- Publisher Link
- http://dx.doi.org/10.1016/j.jhazmat.2017.12.063
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2018
- Description
- A Gram-positive bacterium, Rhodococcus wratislaviensis strain 9, was isolated from groundwater contaminated with nitrophenolics and trichloroethene following enrichment culture technique. The cells of strain 9 grown on LB broth (uninduced) degraded 720 μM p-nitrophenol (PNP) within 12 h, and utilized as a source of carbon and energy. Orthogonal experimental design analysis to determine optimal conditions for biodegradation of PNP showed that pH had a significant positive effect (P ≤ .05) on bacterial degradation of PNP, while glucose, di- and tri-nitrophenols exhibited significant negative effect. Cell-free extracts obtained from PNP-grown culture that contained 20 μg mL⁻¹ protein degraded 90% of 720 μM PNP within 5 h of incubation. Two-dimensional protein analysis revealed differential expression of the oxygenase component of PNP monooxygenase and an elongation factor Tu in PNP-grown cells, but not in those grown on glucose. The strain 9 remediated laboratory wastewater containing 900 μM PNP efficiently within 14 h, indicating its great potential in bioremediation of PNP-contaminated waters.
- Subject
- p-Nitrophenol; Rhodococcus wrastislaviensis; bioremediation; PNP monooxygenase; factor Tu
- Identifier
- http://hdl.handle.net/1959.13/1387226
- Identifier
- uon:32556
- Identifier
- ISSN:0304-3894
- Language
- eng
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